LS Services

Methods Summary Aggregation Analysis AUC Services CD Services LS Services Native Gels Example Applications Further Reading

Click here to learn more about light scattering methods and applicationsAlliance Protein Laboratories has been offering light scattering analysis services since 2001. Tsutomu Arakawa, the President of A.P.L., introduced SEC-MALS to the biotech industry in 1991 after seeing it applied in the laboratory of Dr. Toshio Takagi at Osaka University. He and John Philo (our V.P.) pioneered new methods to analyze conjugated proteins (glycoproteins and PEGylated proteins) and to deconvolute the masses of the peptide and conjugating species. We recently became the first contract laboratory in the US to offer CG-MALS analysis.

A.P.L. has completed hundreds of SEC-MALS and DLS analysis projects on proteins, peptides, nucleic acids, and other molecules. Our APL scientists have over 28 publications in this area.

Further background on SEC-MALS, DLS, CG-MALS and how we apply them can be found on this page.

For SEC-MALS A.P.L. has a Wyatt Technology miniDAWN TREOS MALS light scattering detector used together with both a refractive index detector (Wyatt Optilab rEX) and a UV absorbance detector [see picture of SEC-MALS setup]. For CG-MALS the TREOS MALS detector is used with a Wyatt Calypso and the RI or UV concentration detectors [see picture of CG-MALS setup]. For batch-mode DLS we have a Wyatt Nanostar [picture]. Turn-around for LS analysis is typically 2-3 weeks.

Before even considering using another company for LS analysis see questions you need to ask a biophysical lab.

Below are a few of our publications about light scattering (more can be found on the Further Reading page):

Philo, J. S. (2009). A critical review of methods for size characterization of non-particulate protein aggregates. Curr. Pharm. Biotechnol. 10, 359-372. [A PDF file is available, but by request only (use the Contact form, and be specific about which article you want).]

"Size-Exclusion Chromatography with On-Line Light-Scattering, Absorbance, and Refractive Index Detectors for Studying Proteins and Their Interactions" [review article, 170+ citations] J. Wen, T. Arakawa and J. S. Philo (1996) Anal. Biochem. 240, 155166.

"Aggregation analysis of therapeutic proteins, part 2: Analytical ultracentrifugation and dynamic light scattering". Arakawa, T., Philo, J. S., Ejima, D., Tsumoto, K., and Arisaka, F. (2007). Bioprocess International 5 (4), 36-47.

"Online Size-Exclusion High-Performance Liquid Chromatography Light Scattering and Differential Refractometry Methods to Determine Degree of Polymer Conjugation to Proteins and Protein-Protein or Protein-Ligand Association States", B.S. Kendrick, B.S. Kerwin, B. Chang, and J.S. Philo (2001) Anal. Biochem. 299, 136-146.

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